Anti-TIMM17B antibody (ab122034)
Key features and details
- Rabbit polyclonal to TIMM17B
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIMM17B antibody
See all TIMM17B primary antibodies -
Description
Rabbit polyclonal to TIMM17B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: ILLALIEGVG ILLTRYTAQQ FRNAPPFLED PSQLPPKDGT PAPGYPSYQQ YH, corresponding to C terminal amino acids 121-172 of Human TIMM17B.
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Positive control
- IHC: Human duodenum, placenta, and kidney tissue WB: Lysate from RT4 and U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. -
Tissue specificity
Expression is abundant in heart and skeletal muscle, intermediate in brain, and weak in pancreas, placenta, kidney and liver. -
Sequence similarities
Belongs to the Tim17/Tim22/Tim23 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 10245 Human
- Omim: 300249 Human
- SwissProt: O60830 Human
- Unigene: 30570 Human
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Alternative names
- DXS9822 antibody
- Inner mitochondrial membrane preprotein translocase antibody
- JM3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling TIMM17B with ab122034 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-TIMM17B antibody (ab122034) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) cell lysate -
ab122034 at 1/85 dilution staining TIMM17B in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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ab122034 at 1/85 dilution staining TIMM17B in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122034 has not yet been referenced specifically in any publications.