You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameTIMP1 Mouse ELISA KitSee all TIMP1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 30 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 114 105% - 123%
    Tissue Extracts 106.5 94% - 116%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The ab100745 Mouse TIMP1 (tissue inhibitor of metalloproteinases-1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Mouse TIMP1 cell lysate and tissue lysate. This assay employs an antibody specific for Mouse TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • RelevanceTissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
    • Alternative names
        CLGICollagenase inhibitorEPA
        EPOErythroid potentiating activityFibroblast collagenase inhibitorFLJ90373 HCIHuman Collagenase Inhibitor Metalloproteinase inhibitor 1Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214TIMPTIMP 1 TIMP metallopeptidase inhibitor 1TIMP1 protein Tissue Inhibitor of metalloproteinase 1 Tissue inhibitor of metalloproteinasesTissue inhibitor of metalloproteinases 1Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100745 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      TIMP1 Mouse ELISA Kit images

      • ELISA - TIMP1 Mouse ELISA Kit - 1 x 96 Well Plate (ab100745)
        ELISA - TIMP1 Mouse ELISA Kit - 1 x 96 Well Plate (ab100745)

      Protocols

      References for TIMP1 Mouse ELISA Kit (ab100745)

      ab100745 has not yet been referenced specifically in any publications.

      Publishing research using ab100745 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100745 Submit a Question for ab100745

      Displaying 1 - 1 of 1 results for Abreviews and Q&A

      10
      Votes: Highest First

      I am a PhD student at St Michael's Hospital, Toronto and I have a question about your Mouse TIMP-1 ELISA Kit (ab100744) and your Mouse TIMP-2 ELISA Kit Protocol (ab100746): I only have lung tissue homogenate, and I want to know if your ELISA kits work wit...

      Read More

      Thank you for contacting us. I would suggest using ab100745 TIMP1 Mouse ELISA Kit - 1 x 96 Well Plate. This has been specifically designed for lysate and tissue samples, and optimized to reduce background that can sometimes occur. I have attached so...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"