TIMP1 Mouse ELISA Kit (ab100745)
- Product nameTIMP1 Mouse ELISA KitSee all TIMP1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range24.69 pg/ml - 18000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture extracts 114 105% - 123% Tissue Extracts 106.5 94% - 116%
- Species reactivityReacts with: Mouse
- Product overview
The ab100745 Mouse TIMP1 (tissue inhibitor of metalloproteinases-1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Mouse TIMP1 cell lysate and tissue lysate. This assay employs an antibody specific for Mouse TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent 5x concentrated buffer. For Detection Antibody and HRP-Streptavidin concentrate diluent. 1 x 15ml Detection Antibody TIMP-1: biotinylated anti-mouse TIMP-1 2 vials Sample Diluent Buffer 5x concentrated buffer For Standard/Sample (cell lysate/tissue lysate) diluent. 1 x 10ml Stop Solution Item I 1 x 8ml TIMP1 Microplate (12 strips x 8 wells) coated with anti-Mouse TIMP1 1 unit TIMP1 Standard Mouse (recombinant) 2 vials TMB One-Step Substrate Reagent Item H 1 x 12ml
- CLGICollagenase inhibitorEPA
- EPOErythroid potentiating activityFibroblast collagenase inhibitorFLJ90373 HCIHuman Collagenase Inhibitor Metalloproteinase inhibitor 1Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214TIMPTIMP 1 TIMP metallopeptidase inhibitor 1TIMP1 protein Tissue Inhibitor of metalloproteinase 1 Tissue inhibitor of metalloproteinasesTissue inhibitor of metalloproteinases 1Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
- SwissProt: P12032 Mouse
Our Abpromise guarantee covers the use of ab100745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for TIMP1 Mouse ELISA Kit (ab100745)
ab100745 has not yet been referenced specifically in any publications.