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Overview

  • Product nameTIMP1 Rat ELISA KitSee all TIMP1 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 3000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 92.81 82% - 102%
    Tissue Extracts 93.42 84% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    The biological activities of the proteases are subject to a complex regulation involving specific inhibitors which called TIMP (Tissue inhibitor of metalloproteinases). The mammalian TIMP family includes four members that share structural similarity. Rat TIMP1 is a glycoprotein with a molecular mass of 28-35 kDa produced by a wide range of cell types. TIMP1 plays a major role in modulating the activity of interstitial collagenase as well as a number of connective tissue metalloendoproteases.

    ab100783 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TIMP1 cell lysate and tissue lysate. This assay employs an antibody specific for Rat TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details
  • PlatformMicroplate

Properties

  • RelevanceTissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
    • Alternative names
        CLGICollagenase inhibitorEPA
        EPOErythroid potentiating activityFibroblast collagenase inhibitorFLJ90373 HCIHuman Collagenase Inhibitor Metalloproteinase inhibitor 1Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214TIMPTIMP 1 TIMP metallopeptidase inhibitor 1TIMP1 protein Tissue Inhibitor of metalloproteinase 1 Tissue inhibitor of metalloproteinasesTissue inhibitor of metalloproteinases 1Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100783 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      TIMP1 Rat ELISA Kit images

      • Typical data
        Typical data

        Representative standard curve using ab100763

      Protocols

      References for TIMP1 Rat ELISA Kit (ab100783)

      ab100783 has not yet been referenced specifically in any publications.

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