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BALB/C mice were injected with recombinant human TIMP-1.
Our Abpromise guarantee covers the use of ab1827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20026655|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/50 - 1/100. ABC method. 10 mM citrate buffer, pH 6.0 is recommended for the antigen retrieval step.|
|IHC-Fr||1/50 - 1/100. ABC method.|
|WB||Use at an assay dependent concentration. PubMed: 20026655|
ab1827 - immunohistochemistry
Formalin fixed paraffin embedded human breast carcinoma stained with TIMP1, using ABC and DAB chromogen.
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