Overview

  • Product nameAnti-TIMP1 antibody [102D1]See all TIMP1 primary antibodies ...
  • Description
    Mouse monoclonal [102D1] to TIMP1
  • SpecificityThis antibody is specific to 28.5 kDa protein known as TIMP-1.
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were injected with recombinant human TIMP-1.

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab1827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20026655
IHC-P 1/50 - 1/100. ABC method. 10 mM citrate buffer, pH 6.0 is recommended for the antigen retrieval step.
IHC-Fr 1/50 - 1/100. ABC method.
WB Use at an assay dependent dilution. PubMed: 20026655

Target

  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Clgi antibody
    • Collagenase inhibitor antibody
    • Collagenase inhibitor, Human antibody
    • EPA antibody
    • EPO antibody
    • Erythroid Potentiating Activity antibody
    • Erythroid-potentiating activity antibody
    • Fibroblast collagenase inhibitor antibody
    • FLJ90373 antibody
    • HCI antibody
    • Human Collagenase Inhibitor antibody
    • Metalloproteinase inhibitor 1 antibody
    • Metalloproteinase inhibitor 1 precursor antibody
    • OTTHUMP00000023214 antibody
    • TIMP 1 antibody
    • TIMP antibody
    • TIMP metallopeptidase inhibitor 1 antibody
    • TIMP-1 antibody
    • Timp1 antibody
    • TIMP1 protein antibody
    • TIMP1_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 1 antibody
    • Tissue inhibitor of metalloproteinases 1 antibody
    • Tissue inhibitor of metalloproteinases antibody
    • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
    see all

Anti-TIMP1 antibody [102D1] images

  • ab1827 - immunohistochemistry

    Formalin fixed paraffin embedded human breast carcinoma stained with TIMP1, using ABC and DAB chromogen.

  • Anti-TIMP1 antibody [102D1] (ab1827) at 1 µg/ml + Active human TIMP1 full length protein (ab82104) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 5000 µg/ml
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

References for Anti-TIMP1 antibody [102D1] (ab1827)

This product has been referenced in:
  • Alarab M  et al. Expression of Extracellular Matrix-Remodeling Proteins Is Altered in Vaginal Tissue of Premenopausal Women With Severe Pelvic Organ Prolapse. Reprod Sci N/A:N/A (2013). IHC-Fr ; Human . Read more (PubMed: 24343133) »
  • Egea V  et al. Tissue inhibitor of metalloproteinase-1 (TIMP-1) regulates mesenchymal stem cells through let-7f microRNA and Wnt/ß-catenin signaling. Proc Natl Acad Sci U S A 109:E309-16 (2012). WB ; Human . Read more (PubMed: 22223664) »

See all 6 Publications for this product

Product Wall

We have issued a free of charge replacement.

Merci pour cette confirmation.

Comme convenu j'aimis en place l'envoi de 2 tubes de remplacement pour les ab1827 et ab58803. Le numéro de commande de remplacement gratuit de ce produit est ******. Vous recevrez prochainement un mail de ...

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Merci pour ces précisions.

Après vérification de notre stock,je peux égalementvous envoyer un lot différent pour ab1827 (TIMP1).

Merci de me confirmer que cette résolution vous convient et jep...

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Thanks for completing and submitting the IHC questionnaire. I'm sorry to hear this vial of ab1827 is giving you trouble.

Your protocol looks good.One idea is perhaps a 48hr fixation time is too long, perhaps over fixing the samples and obsscu...

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Thank you for contacting Abcam.


Regarding ab1827 the full length protein was used as an immunogen.


Regarding ab61224, it is a peptide from the central region of the protein. The specific sequence is proprietary.
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Thank you for contacting us.

The immunogen for ab61224 correspond to thecentral region of human TIMP1. The full protein sequence can be found by clicking the link; http://www.uniprot.org/uniprot/P01033

The ab1827 is against the who...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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As shown on the attached pdf, the homology between the human and the mouse TIMP1 proteins is only 72%. Furthermore, the epitope of ab1827 has not been determined and it is a Mouse monoclonal antibody, meaning that the staining in IHC will be Mouse on M...

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Thank you for all the additional information. At this point, I'm still not sure why ab1827 (TIMP1 Ab) did not work in your WB. Could you please let me know if you would like a free of charge replacement of TIMP1 (another lot# of course) or anot...

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