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Anti-TIMP1 antibody [102D1] (ab1827)

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Overview

Product name

Anti-TIMP1 antibody [102D1]
See all TIMP1 products (17) ...

Description

Mouse monoclonal [102D1] to TIMP1

Specificity

This antibody is specific to 28.5 kDa protein known as TIMP-1.

Tested applications

ICC/IF, IHC-P, IHC-Fr, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

BALB/C mice were injected with recombinant human TIMP-1.

Positive control

Breast carcinoma

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

102D1

Isotype

IgG1

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP1 antibody [102D1] (ab1827)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP1 antibody [102D1] (ab1827) image (enlarge)

  • Western blot - Anti-TIMP1 antibody [102D1] (ab1827)Western blot - Anti-TIMP1 antibody [102D1] (ab1827) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 20026655)

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.(ABC...Read more →

    IHC-P: 1/50 - 1/100.(ABC method. 10 mM citrate buffer, pH 6.0 is recommended for the antigen retrieval step.)

  • ShowHide

    IHC-Fr

     IHC-Fr: 1/50 - 1/100.(AB...Read more →

    IHC-Fr: 1/50 - 1/100.(ABC method.)

  • ShowHide1 Image

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. (PubMed: 20026655)

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Post-translational
modifications

The activity of TIMP1 is dependent on the presence of disulfide bonds.

Cellular localization

Secreted.

Target information above from: UniProt accessionP01033 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Clgi antibody
  • Collagenase inhibitor antibody
  • EPA antibody
  • EPO antibody
  • Erythroid Potentiating Activity antibody
  • Erythroid-potentiating activity antibody
  • Fibroblast collagenase inhibitor antibody
  • FLJ90373 antibody
  • HCI antibody
  • Human Collagenase Inhibitor antibody
  • Metalloproteinase inhibitor 1 antibody
  • Metalloproteinase inhibitor 1 precursor antibody
  • OTTHUMP00000023214 antibody
  • TIMP 1 antibody
  • TIMP antibody
  • TIMP metallopeptidase inhibitor 1 antibody
  • TIMP-1 antibody
  • Timp1 antibody
  • TIMP1 protein antibody
  • TIMP1_HUMAN antibody
  • Tissue Inhibitor of Metalloproteinase 1 antibody
  • Tissue inhibitor of metalloproteinases 1 antibody
  • Tissue inhibitor of metalloproteinases antibody
  • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
see all

Database links

Anti-TIMP1 antibody [102D1] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP1 antibody [102D1] (ab1827)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP1 antibody [102D1] (ab1827)

ab1827 - immunohistochemistry

Formalin fixed paraffin embedded human breast carcinoma stained with TIMP1, using ABC and DAB chromogen.

  Western blot - Anti-TIMP1 antibody [102D1] (ab1827)

Western blot - Anti-TIMP1 antibody [102D1] (ab1827)

Anti-TIMP1 antibody [102D1] (ab1827) at 1 µg/ml + TIMP1 protein (Active) (ab82104) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 5000 µg/ml
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 3 minutes

References for Anti-TIMP1 antibody [102D1] (ab1827)

This product has been referenced in:

  • Barker HEet al. LOXL2-mediated matrix remodeling in metastasis and mammary gland involution. Cancer Res 71:1561-72 (2011).Read more (PubMed: 21233336) »
  • von Blume Jet al. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. J Cell Biol 187:1055-69 (2009). WB, ICC/IF; Human.Read more (PubMed: 20026655) »

See all 2 publications for this product

Publishing research using ab1827? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"