TIMP1 antibody [102D1] (ab1827)

Product Name 

TIMP1 antibody [102D1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [102D1] to TIMP1

Immunogen 

BALB/C mice were injected with recombinant human TIMP-1.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific to 28.5 kDa protein known as TIMP-1.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
This antibody may be diluted to a titer of 1:50-1:100 in an ABC method.
We suggest an incubation period of 30 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Positive control 
(see definition)

Breast carcinoma

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

Relevance 

Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B).

TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells.

TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

102D1

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for TIMP1 antibody [102D1] images, references, products related to ab1827 and other tools.

TIMP1 antibody [102D1] images:

References for TIMP1 antibody [102D1] (ab1827)

  This product has been used in:

von Blume J et al. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. J Cell Biol 187:1055-69 (2009). WB, ICC/IF; Human. PubMed: 20026655

If you publish research using ab1827 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding TIMP1 antibody [102D1]

Customer FAQs regarding TIMP1 antibody [102D1]

Protocols for TIMP1 antibody [102D1]

Price and availability of products related to TIMP1 antibody [102D1]

TIMP1 antibody [102D1] - related products: