TIMP1 antibody [2E7.1] (ab28261)

Product Name 

TIMP1 antibody [2E7.1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2E7.1] to TIMP1

Immunogen 

Full length native protein (purified) of Human TIMP1.

Reacts with 
(species key)

Hu

Specificity 

This antibody binds to TIMP-1, but does not cross react with the other TIMP family members (TIMP-2, TIMP-3, TIMP-4).

This product recognizes both native and reduced TIMP1.

We have a range of domain specific antibodies for this target. For a full list please see all TIMP1 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 23 kDa. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell media from human chondrosarcoma cells treated with TPA

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

Relevance 

Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B).

TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells.

TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2E7.1

Isotype 

IgG1

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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