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Our Abpromise guarantee covers the use of ab77848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TIMP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml. (using 100 µl/well antibody solution). Used in conjunction with compatible secondary reagents ab77848 allows the detection of at least 0.2 - 0.4 ng/well of recombinant human TIMP1.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TIMP1 (ab77847). Can be used as detection antibody with recommended pair. Use 100 µl/well antibody solution.|
ab77848 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"