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Overview

  • Product nameAnti-TIMP1 antibody (Biotin)See all TIMP1 primary antibodies ...
  • Description
    Rabbit polyclonal to TIMP1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsWB, ELISA, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human TIMP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab77848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TIMP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA ELISA: Use a concentration of 0.25 - 1 µg/ml. (using 100 µl/well antibody solution). Used in conjunction with compatible secondary reagents ab77848 allows the detection of at least 0.2 - 0.4 ng/well of recombinant human TIMP1.
Sandwich ELISA sELISA: Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TIMP1 (ab77847). Can be used as detection antibody with recommended pair. Use 100 µl/well antibody solution.

Target

  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications    The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01033 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Clgi antibodyCollagenase inhibitor antibodyEPA antibody
        EPO antibodyErythroid Potentiating Activity antibodyErythroid-potentiating activity antibodyFibroblast collagenase inhibitor antibodyFLJ90373 antibodyHCI antibodyHuman Collagenase Inhibitor antibodyMetalloproteinase inhibitor 1 antibodyMetalloproteinase inhibitor 1 precursor antibodyOTTHUMP00000023214 antibodyTIMP 1 antibodyTIMP antibodyTIMP metallopeptidase inhibitor 1 antibodyTIMP-1 antibodyTimp1 antibodyTIMP1 protein antibodyTIMP1_HUMAN antibodyTissue Inhibitor of Metalloproteinase 1 antibodyTissue inhibitor of metalloproteinases 1 antibodyTissue inhibitor of metalloproteinases antibodyTtissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    References for Anti-TIMP1 antibody (Biotin) (ab77848)

    ab77848 has not yet been referenced specifically in any publications.

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