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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Anti-TIMP1 antibody - Carboxyterminal end (ab38978)
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab38978? Please let us know so that we can cite the reference in this datasheet
ab38978 has been referenced in 2 publications.
- Urso ML et al. Adenosine A3 receptor stimulation reduces muscle injury following physical trauma and is associated with alterations in the MMP/TIMP response. J Appl Physiol 112:658-70 (2012). WB; Mouse. PubMed: 22114177
- Didangelos A et al. Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach. Mol Cell Proteomics 10:M111.008128 (2011). WB; Human. PubMed: 21593211
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Sandwich ELISA - TIMP1 antibody - Carboxyterminal end (ab38978)
Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
Western blot - TIMP1 antibody (ab38978)
All lanes : Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution
Lane 1 : Human TIMP1
Lane 2 : Cell media from human chondrosarcoma (no treatment)
Lane 3 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Western blot - TIMP1 antibody - Carboxyterminal end (ab38978)
Predicted band size : 23 kDa
Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
Each lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10µg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.Samples blocked using 5% BSA for 1 hour at 25°C.Non-specific band at 27kDa is thought to be Ig light chain.
Image courtesy of Koji Ueda by Abreview
TIMP1 antibody - Carboxyterminal end for WB in Human (38978)
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