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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Anti-TIMP1 antibody - Carboxyterminal end (ab38978)
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Sandwich ELISA - TIMP1 antibody - Carboxyterminal end (ab38978)
Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
Western blot - TIMP1 antibody (ab38978)
All lanes : Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution
Lane 1 : Human TIMP1
Lane 2 : Cell media from human chondrosarcoma (no treatment)
Lane 3 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Western blot - TIMP1 antibody - Carboxyterminal end (ab38978)
Predicted band size : 23 kDa
Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
Each lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10µg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.Samples blocked using 5% BSA for 1 hour at 25°C.Non-specific band at 27kDa is thought to be Ig light chain.
Image courtesy of Koji Ueda by Abreview
TIMP1 antibody - Carboxyterminal end for WB in Human (38978)
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