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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP1 antibody - Carboxyterminal end
See all TIMP1 products (17) ...
Description
Rabbit polyclonal to TIMP1 - Carboxyterminal end
Specificity
This antibody recognizes both reduced and non reduced TIMP1 protein, but does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4).
Tested applications
ELISA, IP, WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the carboxyterminal region of human TIMP1.
Positive control
WB: Human TIMP1 and cell media from human chondrosarcoma cells treated with TPA.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/5000.Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).(Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 29 kDa when used against the reduced protein. Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection.)
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [2E7.1] to TIMP1 (ab28261). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab28261 as Capture.)
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01033
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- EPA antibody
see all
Database links
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
Anti-TIMP1 antibody - Carboxyterminal end images:
Sandwich ELISA - TIMP1 antibody - Carboxyterminal end (ab38978)
Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
Western blot - TIMP1 antibody (ab38978)
All lanes : Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution
Lane 1 : Human TIMP1
Lane 2 : Cell media from human chondrosarcoma (no treatment)
Lane 3 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Western blot - TIMP1 antibody - Carboxyterminal end (ab38978)
Predicted band size : 23 kDa
Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
Each lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10µg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.Samples blocked using 5% BSA for 1 hour at 25°C.Non-specific band at 27kDa is thought to be Ig light chain.
Image courtesy of Koji Ueda by Abreview
References for Anti-TIMP1 antibody - Carboxyterminal end (ab38978)
This product has been referenced in:
- Didangelos Aet al. Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach. Mol Cell Proteomics 10:M111.008128 (2011). WB; Human.Read more (PubMed: 21593211) »
See 1 publication for this product
Publishing research using ab38978? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"