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Anti-TIMP1 antibody (ab61224)

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Overview

Product name

Anti-TIMP1 antibody
See all TIMP1 products (17) ...

Description

Rabbit polyclonal to TIMP1

Specificity

This antibody has been used successfuly in mouse tissue in a publication, however our WB testing data shows non-reactivity to mouse and we can not guarantee reactivity with mouse.

Tested applications

ELISA, WB, Flow Cyt, IHC-Frmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide derived from the internal region of human TIMP1.

Positive control

293 cell extract, or rat brain lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

  • Western blot - TIMP1 antibody (ab61224)Western blot - TIMP1 antibody (ab61224) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab61224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

  • ShowHide

    Flow Cyt

     Flow Cyt: Use at an assa...Read more →

    Flow Cyt: Use at an assay dependent dilution. (PubMed: 21133852)

  • ShowHide

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution. (PubMed: 21133852)

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Post-translational
modifications

The activity of TIMP1 is dependent on the presence of disulfide bonds.

Cellular localization

Secreted.

Target information above from: UniProt accessionP01033 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Clgi antibody
  • Collagenase inhibitor antibody
  • EPA antibody
  • EPO antibody
  • Erythroid Potentiating Activity antibody
  • Erythroid-potentiating activity antibody
  • Fibroblast collagenase inhibitor antibody
  • FLJ90373 antibody
  • HCI antibody
  • Human Collagenase Inhibitor antibody
  • Metalloproteinase inhibitor 1 antibody
  • Metalloproteinase inhibitor 1 precursor antibody
  • OTTHUMP00000023214 antibody
  • TIMP 1 antibody
  • TIMP antibody
  • TIMP metallopeptidase inhibitor 1 antibody
  • TIMP-1 antibody
  • Timp1 antibody
  • TIMP1 protein antibody
  • TIMP1_HUMAN antibody
  • Tissue Inhibitor of Metalloproteinase 1 antibody
  • Tissue inhibitor of metalloproteinases 1 antibody
  • Tissue inhibitor of metalloproteinases antibody
  • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
see all

Database links

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Anti-TIMP1 antibody images:

  Western blot - TIMP1 antibody (ab61224)

Western blot - TIMP1 antibody (ab61224)

All lanes : Anti-TIMP1 antibody (ab61224) at 1/500 dilution

Lane 1 : extracts from 293 cells
Lane 2 : extracts from 293 cells with the immunizing peptide


Predicted band size : 23 kDa
Observed band size : 23 kDa

References for Anti-TIMP1 antibody (ab61224)

This product has been referenced in:

  • Clark RTet al. T cell production of matrix metalloproteases and inhibition of parasite clearance by TIMP-1 during chronic Toxoplasma infection in the brain. ASN Neuro : (2010). Flow Cyt, IHC-Fr; Mouse.Read more (PubMed: 21133852) »

See 1 publication for this product

Publishing research using ab61224? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"