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ab82104 |
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ab86650 |
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Anti-TIMP1 antibody (ab77099) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Additional bands at : 13 kDa,50 kDa. We are unsure as to the identity of these extra bands.
We hypothesize that the 21 kDa band represents the mature form of TIMP1, after removal of the signal peptide.
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