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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP1 antibody
See all TIMP1 products (17) ...
Description
Rabbit polyclonal to TIMP1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Sheep, Cow, Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 TIMP1.
(Peptide available as ab86650.)
Positive control
This antibody gave a positive signal in HepG2 Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Western blot - TIMP1 antibody (ab77099)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01033
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- EPA antibody
see all
Database links
- Entrez Gene: 282092 Cow
- Entrez Gene: 7076 Human
- Entrez Gene: 443331 Sheep
- Omim: 305370 Human
- SwissProt: P20414 Cow
- SwissProt: P01033 Human
- SwissProt: P50122 Sheep
- Unigene: 522632 Human
Anti-TIMP1 antibody images:
Western blot - TIMP1 antibody (ab77099)
Anti-TIMP1 antibody (ab77099) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Additional bands at : 13 kDa,50 kDa. We are unsure as to the identity of these extra bands.
We hypothesize that the 21 kDa band represents the mature form of TIMP1, after removal of the signal peptide.
References for Anti-TIMP1 antibody (ab77099)
ab77099 has not yet been referenced specifically in any publications.
Publishing research using ab77099? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"