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Western blot - TIMP1 antibody (ab77099)
(enlarge)
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Product Name
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TIMP1 antibody
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See all TIMP1 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TIMP1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 TIMP1.
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Reacts with
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Hu
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Predicted to react
(species key)
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NHuPrm, Shp
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in HepG2 Whole Cell Lysate.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Relevance
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Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B).
TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells.
TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.600 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIMP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TIMP1 antibody images, references, products related to ab77099 and other tools.
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TIMP1 antibody images:
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 Western blot - TIMP1 antibody (ab77099)
TIMP1 antibody (ab77099) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Additional bands at : 13 kDa,50 kDa. We are unsure as to the identity of these extra bands.
We hypothesize that the 21 kDa band represents the mature form of TIMP1, after removal of the signal peptide.
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Search PubMed (MEDLINE) for references to TIMP1
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TIMP1 antibody - more information
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Customer reviews (feedback) regarding TIMP1 antibody |
Customer FAQs regarding TIMP1 antibody |
Protocols for TIMP1 antibody |
Price and availability of products related to TIMP1 antibody |
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TIMP1 antibody - related products:
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TIMP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TIMP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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