![Western blot - Anti-TIMP1 antibody [102D1] (ab1827) image](#TIMP1-Primary-antibodies-ab1827-3.jpg)
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Product name
TIMP1 protein (Active)
See all TIMP1 products (9) ...
Protein description
Recombinant full length Human TIMP1; amino acids 24-207, 20.6kDa. Swiss ID = P01033.
Expression host
E. coli
Properties
Purity
> 95% by SDS-PAGE
Purification notes
Purity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Biological activity
Biological Activity : TIMP1 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP1 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
Form
Lyophilised:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week).
For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
Storage instructions
The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles. See reconstitution notes
Concentration
Concentration information loading...
Sequence notes
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent concentration. Ab82104 can be used as a WB positive control in conjunction with ab1827.
Protein info
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01033
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
TIMP1 protein (Active) images:
Sandwich ELISA - TIMP1 protein (Active) (ab82104)
Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
Western blot - Anti-TIMP1 antibody [102D1] (ab1827)
![Western blot - Anti-TIMP1 antibody [102D1] (ab1827)](/ps/datasheet/images/1/ab1827/TIMP1-Primary-antibodies-ab1827-3.jpg)
Anti-TIMP1 antibody [102D1] (ab1827) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 5000 µg/ml
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 3 minutes
References for TIMP1 protein (Active) (ab82104)
ab82104 has not yet been referenced specifically in any publications.
Publishing research using ab82104? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"