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Overview

  • Product nameTIMP2 Mouse ELISA KitSee all TIMP2 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 80 pg/ml
  • Range
    0.082 ng/ml - 60 ng/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.36 82% - 105%
    Serum 92.65 80% - 112%
    Plasma 93.42 81% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's TIMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP2 in serum, plasma and cell culture supernatants.

     

    This assay employs an antibody specific for mouse TIMP2 coated on a 96-well plate. Standards and samples are pipette into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
    • Alternative names
        CSC 21K CSC21K D11Bwg1104e
        Metalloproteinase inhibitor 2 Metalloproteinase inhibitor 2 precursor MGC105282OTTMUSP00000004170OTTMUSP00000004171RP23-394O9.2TIMP 2 TIMP metallopeptidase inhibitor 2 Tissue inhibitor of metalloproteinase 2Tissue inhibitor of metalloproteinases 2
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100746 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      TIMP2 Mouse ELISA Kit images

      • Representative Standard Curve
        Representative Standard Curve

        Representative Standard Curve using ab100746

      • Representative Standard Curve
        Representative Standard Curve

        Representative Standard Curve using ab100746

      Protocols

      References for TIMP2 Mouse ELISA Kit (ab100746)

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      starting dilution for plasma samples?


      We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"