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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [3A4] (ab1828)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [3A4] (ab1828)](/ps/datasheet/images/ab1828_1.jpg)
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Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)
![Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)](\ps\datasheet\images\1\ab1828\TIMP2-Primary-antibodies-ab1828-7.jpg)
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Product Name
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TIMP2 antibody [3A4]
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See all TIMP2 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3A4] to TIMP2
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Immunogen
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BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Gpig, Rb
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Specificity
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This antibody is specific for TIMP-2 (21 kDa).
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: Use at a concentration of 5 µg/ml. IHC-P: 1/25 - 1/50. This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. IHC-Fr: 1/100. WB: 1/1000.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Colon carcinoma
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Relevance
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Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.
The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP).
TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3A4
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: 1% BSA Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIMP2 antibody [3A4] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TIMP2 antibody [3A4] images, references, products related to ab1828 and other tools.
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TIMP2 antibody [3A4] images:
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [3A4] (ab1828)
ab1828 staining human pancreatic tumour by IHC-P.
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Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)
ICC/IF image of ab1828 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1828, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for TIMP2 antibody [3A4] (ab1828)
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This product has been used in: (two most recent references)
Sbai O et al. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes. Glia 58:344-66 (2010). WB; Mouse. PubMed: 19780201
Maclauchlan S et al. Enhanced angiogenesis and reduced contraction in thrombospondin-2-null wounds is associated with increased levels of matrix metalloproteinases-2 and -9, and soluble VEGF. J Histochem Cytochem 57:301-13 (2009). PubMed: 19029404
See all 5 publication references for this product.
If you publish research using ab1828 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TIMP2
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TIMP2 antibody [3A4] - more information
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TIMP2 antibody [3A4] - related products:
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TIMP2 antibody [3A4] - other tools:
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Contact Abcam with a Technical Enquiry about TIMP2 antibody [3A4] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"