![Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828) image](#TIMP2-Primary-antibodies-ab1828-7.jpg)
![Western blot - TIMP2 antibody [3A4] (ab1828) image](#TIMP2-Primary-antibodies-ab1828-10.jpg)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP2 antibody [3A4]
See all TIMP2 products (16) ...
Description
Mouse monoclonal [3A4] to TIMP2
Specificity
This antibody is specific for TIMP-2 (21 kDa).
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Guinea pig, Cow, Human
Immunogen
BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.
Positive control
Colon carcinoma
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
3A4
Isotype
IgG2a
Light chain type
kappa
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/1000
IHC-P
IHC-P: 1/25 - 1/50.IHC-P: 1/25 - 1/50.
IHC-Fr
IHC-Fr: 1/100IHC-Fr: 1/100
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP16035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Database links
- Entrez Gene: 282093 Cow
- Entrez Gene: 100135629 Guinea pig
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 100008689 Rabbit
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16368 Cow
see all
Anti-TIMP2 antibody [3A4] images:
Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)
![Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)](/ps/datasheet/images/1/ab1828/TIMP2-Primary-antibodies-ab1828-7.jpg)
ICC/IF image of ab1828 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1828, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - TIMP2 antibody [3A4] (ab1828)
![Western blot - TIMP2 antibody [3A4] (ab1828)](/ps/datasheet/images/1/ab1828/TIMP2-Primary-antibodies-ab1828-10.jpg)
Anti-TIMP2 antibody [3A4] (ab109708) at 5 µg/ml + Recombinant TIMP2 at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
Western blot
Anti-TIMP2 antibody [3A4] (ab109708) at 5 µg/ml + Recombinant TIMP2 at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
References for Anti-TIMP2 antibody [3A4] (ab1828)
This product has been referenced in:
- Raaf Let al. Myocardial fibrosis and TGFB expression in hyperhomocysteinemic rats. Mol Cell Biochem 347:63-70 (2011). WB; Rat.Read more (PubMed: 20938722) »
- Sbai Oet al. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes. Glia 58:344-66 (2010). WB; Mouse.Read more (PubMed: 19780201) »
See all 9 publications for this product
Publishing research using ab1828? Please let us know so that we can cite the reference in this datasheet
![TIMP2 antibody [3A4] for WB in Rat (1828)](/ps/Reviews/Images/ab8704_38303.jpg)
![TIMP2 antibody [3A4] for WB in Rat (1828)](/ps/datasheet/images/1/ab1828/TIMP2-Primary-antibodies-ab1828-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"