TIMP2 antibody [3A4] (ab1828)

Product Name 

TIMP2 antibody [3A4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [3A4] to TIMP2

Immunogen 

BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Gpig, Rb

Specificity 

This antibody is specific for TIMP-2 (21 kDa).

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: 1/25 - 1/50. This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr: 1/100.
WB: 1/1000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Colon carcinoma

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

Relevance 

Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.

The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP).

TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3A4

Isotype 

IgG2a

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for TIMP2 antibody [3A4] images, references, products related to ab1828 and other tools.

TIMP2 antibody [3A4] images:

References for TIMP2 antibody [3A4] (ab1828)

  This product has been used in: (two most recent references)

Sbai O et al. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes. Glia 58:344-66 (2010). WB; Mouse. PubMed: 19780201

Maclauchlan S et al. Enhanced angiogenesis and reduced contraction in thrombospondin-2-null wounds is associated with increased levels of matrix metalloproteinases-2 and -9, and soluble VEGF. J Histochem Cytochem 57:301-13 (2009). PubMed: 19029404

See all 5 publication references for this product.


If you publish research using ab1828 please let us know so that we can cite the reference on this datasheet.

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