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Anti-TIMP2 antibody [3A4] (ab1828)

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Overview

Product name

Anti-TIMP2 antibody [3A4]
See all TIMP2 products (16) ...

Description

Mouse monoclonal [3A4] to TIMP2

Specificity

This antibody is specific for TIMP-2 (21 kDa).

Tested applications

ICC/IF, WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Guinea pig, Cow, Human

Immunogen

BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.

Positive control

Colon carcinoma

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

3A4

Isotype

IgG2a

Light chain type

kappa

  • Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828) image (enlarge)

  • Western blot - TIMP2 antibody [3A4] (ab1828)Western blot - TIMP2 antibody [3A4] (ab1828) image (enlarge)

  • Western blot Western blot  image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Post-translational
modifications

The activity of TIMP2 is dependent on the presence of disulfide bonds.

Cellular localization

Secreted.

Target information above from: UniProt accessionP16035 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CSC 21K antibody
  • CSC-21K antibody
  • CSC21K antibody
  • Metalloproteinase inhibitor 2 antibody
  • Metalloproteinase inhibitor 2 precursor antibody
  • TIMP 2 antibody
  • TIMP metallopeptidase inhibitor 2 antibody
  • TIMP-2 antibody
  • TIMP2 antibody
  • TIMP2_HUMAN antibody
  • Tissue Inhibitor of Metalloproteinase 2 antibody
  • Tissue inhibitor of metalloproteinases 2 antibody
see all

Anti-TIMP2 antibody [3A4] images:

  Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)

Immunocytochemistry/ Immunofluorescence - TIMP2 antibody [3A4] (ab1828)

ICC/IF image of ab1828 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1828, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Western blot - TIMP2 antibody [3A4] (ab1828)

Western blot - TIMP2 antibody [3A4] (ab1828)

Anti-TIMP2 antibody [3A4] (ab109708) at 5 µg/ml + Recombinant TIMP2 at 0.1 µg

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 24 kDa (why is the actual band size different from the predicted?)


Exposure time : 20 minutes

  Western blot

Western blot

Anti-TIMP2 antibody [3A4] (ab109708) at 5 µg/ml + Recombinant TIMP2 at 0.1 µg

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 24 kDa (why is the actual band size different from the predicted?)


Exposure time : 20 minutes

References for Anti-TIMP2 antibody [3A4] (ab1828)

This product has been referenced in:

  • Raaf Let al. Myocardial fibrosis and TGFB expression in hyperhomocysteinemic rats. Mol Cell Biochem 347:63-70 (2011). WB; Rat.Read more (PubMed: 20938722) »
  • Sbai Oet al. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes. Glia 58:344-66 (2010). WB; Mouse.Read more (PubMed: 19780201) »

See all 9 publications for this product

Publishing research using ab1828? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"