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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP2 antibody [3A4]
See all TIMP2 products (16) ...
Description
Mouse monoclonal [3A4] to TIMP2
Specificity
This antibody reacts with human TIMP-2. The antibody was tested for cross-reactivity with TIMP-1 and did not cross-react.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Rabbit
Immunogen
Synthetic peptide: DSGNDIYGNPI, corresponding to amino acids 56-66 of Human TIMP2 (Murray et al., 1998).
DSGNDIYGNP I
Positive control
Colon carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
3A4
Isotype
IgG1
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-P
Application notes
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method (5-20 min at full power, 950 W in 0.01 M sodium citrate buffer, pH 6.0)before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP16035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Database links
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 100008689 Rabbit
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16035 Human
- SwissProt: P25785 Mouse
- SwissProt: Q9TRZ7 Rabbit
see all
References for Anti-TIMP2 antibody [3A4] (ab2465)
This product has been referenced in:
- Murray GIet al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998).Read more (PubMed: 9824606) »
See 1 publication for this product
Publishing research using ab2465? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"