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- Proteins and Peptides
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Product name
Anti-TIMP2 antibody - Carboxyterminal end
See all TIMP2 products (16) ...
Description
Rabbit polyclonal to TIMP2 - Carboxyterminal end
Specificity
The antibody binds to TIMP2, but does not cross react with the other TIMP family members (TIMP1, TIMP3, TIMP4).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Goat, Human
Immunogen
Synthetic peptide based on the carboxyterminal end of human TIMP2.
(Peptide available as ab452 53.)
Positive control
WB: Human TIMP2 and cell media from human chondrosarcoma (untreated).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Western blot - TIMP2 antibody (ab38975)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: A recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 22 kDa when used against the reduced protein (predicted molecular weight: 24 kDa). Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP16035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Database links
- Entrez Gene: 7077 Human
- Omim: 188825 Human
- SwissProt: P16035 Human
- Unigene: 633514 Human
Anti-TIMP2 antibody - Carboxyterminal end images:
Western blot - TIMP2 antibody (ab38975)
All lanes : Anti-TIMP2 antibody - Carboxyterminal end (ab38975) at 1/1000 dilution
Lane 1 : Human TIMP2
Lane 2 : Cell media from human chondrosarcoma (untreated)
Predicted band size : 24 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-TIMP2 antibody - Carboxyterminal end (ab38975)
ab38975 has not yet been referenced specifically in any publications.
Publishing research using ab38975? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"