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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab38973? Please let us know so that we can cite the reference in this datasheet
ab38973 has been referenced in 3 publications.
- Dixon JA et al. Targeted regional injection of biocomposite microspheres alters post-myocardial infarction remodeling and matrix proteolytic pathways. Circulation 124:S35-45 (2011). PubMed: 21911817
- Jones JA et al. Alterations in membrane type-1 matrix metalloproteinase abundance after the induction of thoracic aortic aneurysm in a murine model. Am J Physiol Heart Circ Physiol 299:H114-24 (2010). WB; Mouse. PubMed: 20418476
- Dixon JA et al. Mesenchymal cell transplantation and myocardial remodeling after myocardial infarction. Circulation 120:S220-9 (2009). PubMed: 19752372
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - TIMP2 antibody (ab38973)
All lanes : Anti-TIMP2 antibody - Loop 1 (ab38973) at 1/1000 dilution
Lane 1 : Human TIMP2
Lane 2 : Mouse TIMP2
Lane 3 : Cell media from human chondrosarcoma (untreated)
Predicted band size : 24 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP2 antibody - Loop 1(ab38973)
Ab38973 staining Human placenter. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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