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Anti-TIMP2 antibody - Loop 2 (ab28260)

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Overview

Product name

Anti-TIMP2 antibody - Loop 2
See all TIMP2 products (16) ...

Description

Rabbit polyclonal to TIMP2 - Loop 2

Specificity

This antibody binds to TIMP2, but does not cross react with the other TIMP family members (TIMP1, TIMP3, TIMP4).

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide based on loop 2 of human TIMP2.

Read Abcam's proprietary immunogen policy

(Peptide available as ab41151.)

General notes

This antibody is made against loop 2 of human TIMP2, which may be more bioavailable than the amino terminal or carboxy terminal locations.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

  • Western blot - TIMP2 antibody (ab28260)Western blot - TIMP2 antibody (ab28260) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 23 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Post-translational
modifications

The activity of TIMP2 is dependent on the presence of disulfide bonds.

Cellular localization

Secreted.

Target information above from: UniProt accessionP16035 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CSC 21K antibody
  • CSC-21K antibody
  • CSC21K antibody
  • Metalloproteinase inhibitor 2 antibody
  • Metalloproteinase inhibitor 2 precursor antibody
  • Metalloproteinase inhibitor 2 precursor antibody
  • TIMP 2 antibody
  • TIMP 2 antibody
  • TIMP metallopeptidase inhibitor 2 antibody
  • TIMP-2 antibody
  • TIMP2 antibody
  • TIMP2_HUMAN antibody
  • Tissue Inhibitor of Metalloproteinase 2 antibody
  • Tissue Inhibitor of Metalloproteinase 2 antibody
  • Tissue inhibitor of metalloproteinases 2 antibody
see all

Database links

Anti-TIMP2 antibody - Loop 2 images:

  Western blot - TIMP2 antibody (ab28260)

Western blot - TIMP2 antibody (ab28260)

All lanes : Anti-TIMP2 antibody - Loop 2 (ab28260)

Lane 1 : Human TIMP2
Lane 2 : Mouse TIMP2
Lane 3 : Cell media from human chondrosarcoma (untreated)


Predicted band size : 23 kDa
Observed band size : 23 kDa

Protocols

  • Recommended WB protocol

    • References for Anti-TIMP2 antibody - Loop 2 (ab28260)

    This product has been referenced in:

    • Barnes BRet al. Alterations in mRNA and protein levels of metalloproteinases-2, -9, and -14 and tissue inhibitor of metalloproteinase-2 responses to traumatic skeletal muscle injury. Am J Physiol Cell Physiol 297:C1501-8 (2009).Read more (PubMed: 19794148) »

    See 1 publication for this product

    Publishing research using ab28260? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"