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Anti-TIMP2 antibody [SPM356] - Aminoterminal end, prediluted (ab54443)

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Overview

Product name

Anti-TIMP2 antibody [SPM356] - Aminoterminal end, prediluted
See all TIMP2 products (16) ...

Description

Mouse monoclonal [SPM356] to TIMP2 - Aminoterminal end, prediluted

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Rabbit, Guinea pig, Cow

Immunogen

Synthetic peptide from the N-terminal region of human TIMP2.

Epitope

ab54443 reacts with an epitope located in the N-terminus region of TIMP 2.

Positive control

Conditioned, serum-free medium from (TPA-treated) human fetal lung cell line HFL-1. Bladder, breast, and ovarian carcinoma tissue.

Properties

Form

Prediluted

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

SPM356

Isotype

IgG2a

Light chain type

kappa

Research areas

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [SPM356], prediluted (ab54443)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [SPM356], prediluted (ab54443) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Ready-to-use for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Post-translational
modifications

The activity of TIMP2 is dependent on the presence of disulfide bonds.

Cellular localization

Secreted.

Target information above from: UniProt accessionP16035 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CSC 21K antibody
  • CSC-21K antibody
  • CSC21K antibody
  • Metalloproteinase inhibitor 2 antibody
  • Metalloproteinase inhibitor 2 precursor antibody
  • TIMP 2 antibody
  • TIMP metallopeptidase inhibitor 2 antibody
  • TIMP-2 antibody
  • TIMP2 antibody
  • TIMP2_HUMAN antibody
  • Tissue Inhibitor of Metalloproteinase 2 antibody
  • Tissue inhibitor of metalloproteinases 2 antibody
see all

Database links

see all

Anti-TIMP2 antibody [SPM356] - Aminoterminal end, prediluted images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [SPM356], prediluted (ab54443)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIMP2 antibody [SPM356], prediluted (ab54443)

Ab54443 staining human TIMP2 in human pancreatic tumor tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References for Anti-TIMP2 antibody [SPM356] - Aminoterminal end, prediluted (ab54443)

ab54443 has not yet been referenced specifically in any publications.

Publishing research using ab54443? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"