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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP2 antibody
See all TIMP2 products (16) ...
Description
Rabbit polyclonal to TIMP2
Specificity
ab53730 detects endogenous levels of total TIMP2 protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from human TIMP2.
Positive control
A549 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53730 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Western blot - TIMP2 antibody (ab53730)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP16035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Database links
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16035 Human
- SwissProt: P25785 Mouse
- SwissProt: P30121 Rat
- Unigene: 633514 Human
see all
Anti-TIMP2 antibody images:
Western blot - TIMP2 antibody (ab53730)
All lanes : Anti-TIMP2 antibody (ab53730) at 1/500 dilution
Lane 1 : A549 cell extract
Lane 2 : A549 cell extract with immunising peptide
Predicted band size : 24 kDa
Observed band size : 31 kDa (why is the actual band size different from the predicted?)
References for Anti-TIMP2 antibody (ab53730)
This product has been referenced in:
- Fortin CFet al. MT6-MMP is present in lipid rafts and faces inward in living human PMNs but translocates to the cell surface during neutrophil apoptosis. Int Immunol : (2010).Read more (PubMed: 20501611) »
See 1 publication for this product
Publishing research using ab53730? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"