Feedback Form

Western blot - TIMP2 antibody (ab64040)
Western blot - TIMP2 antibody (ab64040)
(enlarge)


Product Name 

TIMP2 antibody

 

See all TIMP2 antibodies (16)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TIMP2

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human TIMP2.

Reacts with 

Rat

Predicted to react 
(species key)

Hu, Ms
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in Rat Skeletal Muscle Tissue Lysate.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

Relevance 

Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.

The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP).

TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

 

Alternative names TIMP2 antibody (ab64040)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

7077    (Human)
21858    (Mouse)
29543    (Rat)

Omim

    

188825    (Human)

SwissProt

    

P16035    (Human)
P25785    (Mouse)
P30121    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIMP2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for TIMP2 antibody images, references, products related to ab64040 and other tools.

 

TIMP2 antibody images:

Product image  Western blot - TIMP2 antibody (ab64040)

Western blot - TIMP2 antibody (ab64040)

TIMP2 antibody (ab64040) at 1 µg/ml + Skeletal Muscle (Rat) Tissue Lysate at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to TIMP2

 

TIMP2 antibody - more information

Customer reviews (feedback) regarding TIMP2 antibody

Customer FAQs regarding TIMP2 antibody

Protocols for TIMP2 antibody

Price and availability of products related to TIMP2 antibody

 

TIMP2 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for TIMP2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other TIMP2 Antibodies

See all TIMP2 antibodies (16)...

 

TIMP2 antibody - other tools:

Contact Abcam with a Technical Enquiry about TIMP2 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.