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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP2 antibody
See all TIMP2 products (16) ...
Description
Rabbit polyclonal to TIMP2
Tested applications
Cross reactivity
Reacts with
Rat
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human TIMP2.
(Peptide available as ab86642.)
Positive control
This antibody gave a positive signal in Rat Skeletal Muscle Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Western blot - TIMP2 antibody (ab64040)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP16035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Database links
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16035 Human
- SwissProt: P25785 Mouse
- SwissProt: P30121 Rat
- Unigene: 633514 Human
see all
Anti-TIMP2 antibody images:
Western blot - TIMP2 antibody (ab64040)
Anti-TIMP2 antibody (ab64040) at 1 µg/ml + Skeletal Muscle (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.
References for Anti-TIMP2 antibody (ab64040)
ab64040 has not yet been referenced specifically in any publications.
Publishing research using ab64040? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"