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Overview

  • Product nameTIMP2 protein (Active)See all TIMP2 proteins and peptides ...
  • Protein descriptionRecombinant full length Human TIMP2; amino acids 27-220 (194), 21.8kDa. Swiss ID = P16035.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Biological activityBiological Activity : TIMP2 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP2 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
  • FormLyophilised
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Sequence notesMCSCSPVHPQ QAFCNADVVI RAKAVSEKEV DSGNDIYGNP IKRIQYEIKQ IKMFKGPEKD IEFIYTAPSS AVCGVSLDVG GKKEYLIAGK AEGDGKMHIT LCDFIVPWDT LSTTQKKSLN HRYQMGCECK ITRCPMIPCY ISSPDECLWM DWVTEKNING HQAKFFACIK RSDGSCAWYR GAAPPKQEFL DIEDP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab82117 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab2465 and ab64040.

    • Protein info

    • Alternative names
        CSC 21KCSC-21KCSC21K
        Metalloproteinase inhibitor 2Metalloproteinase inhibitor 2 precursorTIMP 2TIMP metallopeptidase inhibitor 2TIMP-2TIMP2TIMP2_HUMANTissue Inhibitor of Metalloproteinase 2Tissue inhibitor of metalloproteinases 2
      see all
  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P16035 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for TIMP2 protein (Active) (ab82117)

    ab82117 has not yet been referenced specifically in any publications.

    Publishing research using ab82117 ? Please let us know so that we can cite the reference in this datasheet

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    reconstitution instructions for lot 954191

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    Please find the reconstitution instructions for your lot of ab82117 below:

    Reconstitution:

    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"