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Overview

  • Product nameTIMP2 protein (Mouse)See all TIMP2 proteins and peptides ...
  • Protein descriptionFull length Mouse TIMP2 protein (purified from mouse lung fibroblasts).
  • Expression hostNative

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesThis protein is purified from BHK cells transfected with full-length mouse TIMP-2, and is essentially free of contaminating MMPs or other known TIMPs.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab39315 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Produces a band of approximately 22 kDa, corresponding to the non-glycolisated form. This protein can also be used as a positive control in substrate gel analysis (reverse zymograms).
    ELISA ELISA: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        CSC 21KCSC-21KCSC21K
        Metalloproteinase inhibitor 2Metalloproteinase inhibitor 2 precursorMetalloproteinase inhibitor 2 precursorTIMP 2TIMP 2TIMP metallopeptidase inhibitor 2TIMP-2TIMP2TIMP2_HUMANTissue Inhibitor of Metalloproteinase 2Tissue Inhibitor of Metalloproteinase 2Tissue inhibitor of metalloproteinases 2
      see all
  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P16035 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for TIMP2 protein (Mouse) (ab39315)

    This product has been referenced in:
    • Lluri G  et al. Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro. Exp Cell Res 314:11-24 (2008). Read more (PubMed: 17678891) »

    See 1 Publication for this product

    Publishing research using ab39315 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Inquiry: I'm looking for a positive control for ab1828 (anti-Timp2) to use for western blot. Will TIMP2 protein (mouse) ab39315 work as a positive control for ab1828 (anti-Timp2) in western blot?


    Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
    There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of approx...

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    I just ordered the ab19345 because we need to use it as a normal antibody for WB anyway. Another question is about the TIMP2 protein (ab39315), it is purified from mouse lung fibroblasts, I am wondering whether I can use the protein for functional ass...

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    Thank you for your enquiry and interest in our products. I can confirm that TIMP2 protein was isolated from mouse lung fibroblasts so the amino acid sequence corresponds to the murineTIMP2. According to the datasheet ab39315 has not been tested for fu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"