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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab58804? Please let us know so that we can cite the reference in this datasheet
ab58804 has been referenced in 1 publications.
- Qipshidze N et al. Folic acid mitigated cardiac dysfunction by normalizing the levels of tissue inhibitor of metalloproteinase and homocysteine-metabolizing enzymes postmyocardial infarction in mice. Am J Physiol Heart Circ Physiol 299:H1484-93 (2010). WB, ICC/IF; Mouse. PubMed: 20802128
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
](/ps/datasheet/images/58/ab58804/TIMP3-Primary-antibodies-ab58804-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP3 antibody [136-13H4](ab58804)
ab58804 (1µg/ml) staining TIMP3 in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic/secreted staining of syncytiotrophoblasts.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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