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If your product does not perform as described on this datasheet, we will refund or replace your product...
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](/ps/datasheet/images/58/ab58804/TIMP3-Primary-antibodies-ab58804-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP3 antibody [136-13H4](ab58804)
ab58804 (1µg/ml) staining TIMP3 in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic/secreted staining of syncytiotrophoblasts.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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