 image](#TIMP3-Primary-antibodies-ab58804-1.jpg)
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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Granzymes
Overview
Product name
Anti-TIMP3 antibody [136-13H4]
See all TIMP3 products (11) ...
Description
Mouse monoclonal [136-13H4] to TIMP3
Specificity
Recognizes the ~27 kDa glycosylated and the ~24 kDa non-glycosylated forms of TIMP3. Does not cross-react with TIMP1 or TIMP2.
Tested applications
IHC-P, WB, IHC-Frmore details
Cross reactivity
Reacts with
Rabbit, Human, Baboon
Predicted to work with
Mouse, Horse, Xenopus laevis, Cynomolgus Monkey, Macaque Monkey
Does not react with
Rat, Chicken, Cow, Pig
Immunogen
Synthetic peptide: SNFGYPGYQSKHYACIRQK, corresponding to amino acids 170-188 of Human TIMP3
SNFGYPGYQS KHYACIRQK
Positive control
293 or Caco-2 cells Unfixed eye tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.1% BSA, 100mM Sodium phosphate buffer, pH 7.0
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
136-13H4
Myeloma
NS1
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Cell Biology >> Apoptosis >> Extracellular Signals >> Granzymes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP3 antibody [136-13H4](ab58804)
 image](/ps/datasheet/images/58/ab58804/TIMP3-Primary-antibodies-ab58804-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab58804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 5 µg/mlPredicted molecular weight: 24 kDa.
IHC-Fr: Use a concentration of 2 - 10 µg/ml.
Application notes
Is unsuitable for ,ICC/IF or IP.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Involvement in disease
Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP35625
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HSMRK222 antibody
- K222 antibody
- K222TA2 antibody
see all
Database links
- Entrez Gene: 7078 Human
- Entrez Gene: 21859 Mouse
- Entrez Gene: 373596 Xenopus laevis
- Omim: 188826 Human
- SwissProt: Q9TUL9 Horse
- SwissProt: P35625 Human
- SwissProt: P39876 Mouse
- SwissProt: O73746 Xenopus laevis
see all
Anti-TIMP3 antibody [136-13H4] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP3 antibody [136-13H4](ab58804)
ab58804 (1µg/ml) staining TIMP3 in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic/secreted staining of syncytiotrophoblasts.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-TIMP3 antibody [136-13H4] (ab58804)
This product has been referenced in:
- Qipshidze Net al. Folic acid mitigated cardiac dysfunction by normalizing the levels of tissue inhibitor of metalloproteinase and homocysteine-metabolizing enzymes postmyocardial infarction in mice. Am J Physiol Heart Circ Physiol 299:H1484-93 (2010). WB, ICC/IF; Mouse.Read more (PubMed: 20802128) »
See 1 publication for this product
Publishing research using ab58804? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"