Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Granzymes
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ab41152 |
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ab39184 has been referenced in 7 publications.
Publishing research using ab39184? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-TIMP3 antibody - Loop 1 (ab39184)
Lane 1 : Human TIMP3
Lane 2 : Mouse TIMP3
Lane 3 : crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)
Predicted band size : 24 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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