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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Granzymes
Overview
Product name
Anti-TIMP3 antibody - Loop 1
See all TIMP3 products (11) ...
Description
Rabbit polyclonal to TIMP3 - Loop 1
Specificity
Ab39184 binds to TIMP3. It recognizes the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. Ab39184 does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4).
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide of TIMP3 based on loop #1 of the human TIMP3.
(Peptide available as ab41152.)
Positive control
Human and mouse TIMP3 recombinant protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Cell Biology >> Apoptosis >> Extracellular Signals >> Granzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/1000 - 1/5000.Detects a band of approximately 24 , 30 kDa (predicted molecular weight: 24 kDa).(Dilution optimised using Chromogenic detection.)
IHC-P: Use at an assay dependent dilution. (PubMed: 20448060)
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Involvement in disease
Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP35625
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HSMRK222 antibody
- K222 antibody
- K222TA2 antibody
see all
Database links
- Entrez Gene: 7078 Human
- Entrez Gene: 21859 Mouse
- Omim: 188826 Human
- SwissProt: P35625 Human
- SwissProt: P39876 Mouse
- Unigene: 644633 Human
- Unigene: 714168 Human
- Unigene: 4871 Mouse
Anti-TIMP3 antibody - Loop 1 images:
Western blot - TIMP3 antibody - Loop 1 (ab39184)
All lanes : Anti-TIMP3 antibody - Loop 1 (ab39184)
Lane 1 : Human TIMP3
Lane 2 : Mouse TIMP3
Lane 3 : crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)
Predicted band size : 24 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - TIMP3 antibody - Loop 1 (ab39184)
ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-TIMP3 antibody - Loop 1 (ab39184)
This product has been referenced in:
- Lozito TP & Tuan RS Mesenchymal stem cells inhibit both endogenous and exogenous MMPs via secreted TIMPs. J Cell Physiol 226:385-96 (2011). WB; Human.Read more (PubMed: 20665704) »
- Bernot Det al. Down-regulation of tissue inhibitor of metalloproteinase-3 (TIMP-3) expression is necessary for adipocyte differentiation. J Biol Chem 285:6508-14 (2010).Read more (PubMed: 20056610) »
See all 7 publications for this product
Publishing research using ab39184? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"