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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP4 antibody - Loop 2
See all TIMP4 products (7) ...
Description
Rabbit polyclonal to TIMP4 - Loop 2
Specificity
ab38983 recognises TIMP4. It does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP3).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on loop 2 of human TIMP4
(Peptide available as ab41158.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Western blot - TIMP4 antibody (ab38983)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 25 kDa. But note that when used against the reduced protein, ab38983 identifies bands at 23 and 29 Kd, representing the unglycosylated and glycosylated forms respectively of TIMP4. Also note that treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Tissue specificity
Abundant in heart and present at low levels in many other tissues.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ99727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
Database links
- Entrez Gene: 7079 Human
- Omim: 601915 Human
- SwissProt: Q99727 Human
- Unigene: 190787 Human
- Unigene: 591665 Human
Anti-TIMP4 antibody - Loop 2 images:
Western blot - TIMP4 antibody (ab38983)
Anti-TIMP4 antibody - Loop 2 (ab38983) at 1/1000 dilution + human TIMP4
Predicted band size : 25 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-TIMP4 antibody - Loop 2 (ab38983)
ab38983 has not yet been referenced specifically in any publications.
Publishing research using ab38983? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"