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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP4 antibody - Loop 3
See all TIMP4 products (7) ...
Description
Rabbit polyclonal to TIMP4 - Loop 3
Specificity
Ab38984 recognises the loop 3 region of TIMP4. It does not react with other TIMPs.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on loop 3 of Human TIMP4.
(Peptide available as ab41157.)
Positive control
Human TIMP4.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 25 kDa. When used against the reduced protein, ab38984 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Note: Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Tissue specificity
Abundant in heart and present at low levels in many other tissues.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ99727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
Database links
- Entrez Gene: 7079 Human
- Omim: 601915 Human
- SwissProt: Q99727 Human
- Unigene: 190787 Human
- Unigene: 591665 Human
Protocols
References for Anti-TIMP4 antibody - Loop 3 (ab38984)
ab38984 has not yet been referenced specifically in any publications.
Publishing research using ab38984? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"