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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP4 antibody
See all TIMP4 products (7) ...
Description
Rabbit polyclonal to TIMP4
Specificity
Does not cross-react with other TIMP family members (TIMP1, TIMP2, TIMP3).
Tested applications
ELISA, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) C term.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
IP
Application notes
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects bands of approximately 23 and 29 kDa, corresponding to unglycosylated and glycosylated protein.
Note: TIMP4 is reported in heart tissue, and is produced by some fibroblasts in cell culture conditions. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types, but the low protein levels produced often require concentration of the cell culture media to visualize the bands by immunoblotting.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Tissue specificity
Abundant in heart and present at low levels in many other tissues.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ99727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
Database links
- Entrez Gene: 7079 Human
- Omim: 601915 Human
- SwissProt: Q99727 Human
- Unigene: 190787 Human
- Unigene: 591665 Human
References for Anti-TIMP4 antibody (ab11818)
ab11818 has not yet been referenced specifically in any publications.
Publishing research using ab11818? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"