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Anti-TIMP4 antibody (ab58425)

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Overview

Product name

Anti-TIMP4 antibody
See all TIMP4 products (7) ...

Description

Rabbit polyclonal to TIMP4

Specificity

ab58425 detects endogenous levels of total TIMP4 protein.

Tested applications

ELISA, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide derived from C terminal residues of human TIMP4

Positive control

IHC-P: Human lung carcinoma tissue. WB: NIH3T3 cell extracts.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP

  • Western blot - TIMP4 antibody (ab58425)Western blot - TIMP4 antibody (ab58425) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - TIMP4 antibody (ab58425)Immunohistochemistry (Paraffin-embedded sections) - TIMP4 antibody (ab58425) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

Tissue specificity

Abundant in heart and present at low levels in many other tissues.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Cellular localization

Secreted.

Target information above from: UniProt accessionQ99727 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Metalloproteinase inhibitor 4 antibody
  • TIMP 4 antibody
  • TIMP metallopeptidase inhibitor 4 antibody
  • TIMP-4 antibody
  • Timp4 antibody
  • TIMP4_HUMAN antibody
  • Tissue inhibitor of metalloproteinase 4 antibody
  • Tissue inhibitor of metalloproteinases 4 antibody
see all

Database links

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Anti-TIMP4 antibody images:

  Western blot - TIMP4 antibody (ab58425)

Western blot - TIMP4 antibody (ab58425)

All lanes : Anti-TIMP4 antibody (ab58425) at 1/500 dilution

Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 cell extract with immunising peptide


Predicted band size : 26 kDa
Observed band size : 26 kDa

  Immunohistochemistry (Paraffin-embedded sections) - TIMP4 antibody (ab58425)

Immunohistochemistry (Paraffin-embedded sections) - TIMP4 antibody (ab58425)

ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.

References for Anti-TIMP4 antibody (ab58425)

This product has been referenced in:

  • Tong Wet al. Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats. Am J Physiol Heart Circ Physiol 301:H2113-21 (2011).Read more (PubMed: 21856922) »
  • Qipshidze Net al. Folic acid mitigated cardiac dysfunction by normalizing the levels of tissue inhibitor of metalloproteinase and homocysteine-metabolizing enzymes postmyocardial infarction in mice. Am J Physiol Heart Circ Physiol 299:H1484-93 (2010). ICC/IF; Mouse.Read more (PubMed: 20802128) »

See all 3 publications for this product

Publishing research using ab58425? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"