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Products:Cell Biology >> Apoptosis >> Nucleus >> Lamins
Overview
Product name
Anti-TIP30 antibody
See all TIP30 products (3) ...
Description
Goat polyclonal to TIP30
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: AETEALSKLREDFR, corresponding to N terminal amino acids 2-15 of Human TIP30.
AETEALSKLR EDFR-C
Positive control
HeLa and human lung lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
TIP30, also called CC3 or HTATIP2, is a putative metastasis suppressor that promotes apoptosis and inhibits angiogenesis. Although TIP30 has several characteristic features of a tumor suppressor in in vitro analyses, tumor development as a result of TIP30 inactivation has not been demonstrated in vivo, and abnormal expression of TIP30 in human cancer has not been reported.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Apoptosis >> Nucleus >> Lamins
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Western blot - TIP30 antibody (ab10268)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Target
Function
Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
Tissue specificity
Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
Domain
Unique C-terminus confers high proteasome-dependent instability to isoform 2.
Cellular localization
Cytoplasm. Nucleus envelope.
Target information above from: UniProt accessionQ9BUP3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 30 kDa HIV 1 TAT interacting protein antibody
- 30 kDa HIV-1 TAT-interacting protein antibody
- 30 kDa HIV1 TAT interacting protein antibody
see all
Database links
- Entrez Gene: 10553 Human
- Omim: 605628 Human
- SwissProt: Q9BUP3 Human
- Unigene: 90753 Human
Anti-TIP30 antibody images:
Western blot - TIP30 antibody (ab10268)
Predicted band size : 28 kDa
ab10268 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
References for Anti-TIP30 antibody (ab10268)
ab10268 has not yet been referenced specifically in any publications.
Publishing research using ab10268? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"