Overview

  • Product nameAnti-TLE4 antibodySee all TLE4 primary antibodies ...
  • Description
    Rabbit polyclonal to TLE4
  • Tested applicationsELISA, WB, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human TLE4.

  • Positive control
    • Extracts from Jurkat, 293 or HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
IHC-Fr 1/100.

Target

  • RelevanceTLE4 (Transducin-like enhancer protein 4) is a member of the Groucho family of transcriptional corepressors and inhibits the transcriptional activation mediated by Pax5 and by beta-catenin and TCF family members in Wnt signaling.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • B lymphocyte gene 1 antibody
    • E(sp1) homolog Drosophila antibody
    • E(spI) antibody
    • enhancer of split groucho 4 antibody
    • ESG antibody
    • ESG4 antibody
    • ESG4 antibody
    • GRG4 antibody
    • KIAA1261 antibody
    • TIM antibody
    • TIM1 antibody
    • timeless (Drosophila) homolog antibody
    • TLE4 antibody
    • Transducin like enhancer protein 4 antibody
    • transducin-like enhancer of split 4 antibody
    • transducin-like enhancer of split 4 antibody
    see all

Anti-TLE4 antibody images

  • All lanes : Anti-TLE4 antibody (ab64833) at 1/500 dilution

    Lane 1 : Jurkat cell extract at 30 µg
    Lane 2 : 293 cell extract at 30 µg
    Lane 3 : HuvEc cell extract at 30 µg
    Lane 4 : HuvEc cell extract immunising peptide at 10 µg


    Predicted band size : 84 kDa
    Observed band size : 84 kDa
  • ab64833 staining TLE4 in mouse (E16.5) embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde, permeabilized in 0.3% Triton X-100 and blocked in 0.2% Gelatin for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with sample for 24 hours at 4°C. A Cy5® conjugated donkey polyclonal to rabbit IgG, diluted 1/500 was used as secondary.

    See Abreview

References for Anti-TLE4 antibody (ab64833)

This product has been referenced in:
  • Bandyopadhyay S  et al. Tle4 regulates epigenetic silencing of gamma interferon expression during effector T helper cell tolerance. Mol Cell Biol 34:233-45 (2014). Read more (PubMed: 24190972) »

See 1 Publication for this product

Product Wall

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Unfortunately,in this case, the immunogen peptide is not available for sale.

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Read More
Application Western blot
Sample Human Cell lysate - whole cell (MCF7 Human breast cancer cell line)
Specification MCF7 Human breast cancer cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 20°C
Username

Dr. Christine Blancher

Verified customer

Submitted Jul 27 2009

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic brain E16.5)
Specification Embryonic brain E16.5
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X100
Blocking step Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"