![Western blot - TLK1 antibody [EPR2886] (ab108987) image](#TLK1-Primary-antibodies-ab108987-1.jpg)
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Product name
Anti-TLK1 antibody [EPR2886]
See all TLK1 products (6) ...
Description
Rabbit monoclonal [EPR2886] to TLK1
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in Human TLK1 (UniProt Q9UKI8).
Positive control
HeLa and A431 cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2886
Isotype
IgG1
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Western blot - TLK1 antibody [EPR2886] (ab108987)
![Western blot - TLK1 antibody [EPR2886] (ab108987) image](/ps/datasheet/images/108/ab108987/TLK1-Primary-antibodies-ab108987-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab108987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 87 kDa).
IP
IP: 1/10 - 1/100.IP: 1/10 - 1/100.
Flow Cyt: 1/100 - 1/500.
Application notes
Is unsuitable for ,ICC or IHC-P.
Target
Function
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
Tissue specificity
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UKI8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- PKU beta antibody
- PKU-beta antibody
- serine threonine protein kinase antibody
see all
Database links
- Entrez Gene: 9874 Human
- Omim: 608438 Human
- SwissProt: Q9UKI8 Human
- Unigene: 726093 Human
Anti-TLK1 antibody [EPR2886] images:
Western blot - TLK1 antibody [EPR2886] (ab108987)
All lanes : Anti-TLK1 antibody [EPR2886] (ab108987) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size : 87 kDa
Observed band size : 86 kDa (why is the actual band size different from the predicted?)
References for Anti-TLK1 antibody [EPR2886] (ab108987)
ab108987 has not yet been referenced specifically in any publications.
Publishing research using ab108987? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"