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All lanes : Anti-TLK2 antibody (ab74335) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 88 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Detection of TLK2 by Western Blot of Immunprecipitate.
ab74335 at 1µg/ml staining TLK2 in 293 whole cell lysate immunoprecipitated using ab74335 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
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