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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-TLK2 antibody
See all TLK2 products (3) ...
Description
Rabbit polyclonal to TLK2
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 722 and 772 of human TLK2 (EAW94344.1)
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab74335 was affinity purified using an epitope specific to TLK2 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromatin assembly
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Predicted molecular weight: 88 kDa.
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
Tissue specificity
Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ86UE8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC44450 antibody
- PKU alpha antibody
- PKU-alpha antibody
see all
Database links
- Entrez Gene: 11011 Human
- Omim: 608439 Human
- SwissProt: Q86UE8 Human
- Unigene: 445078 Human
Anti-TLK2 antibody images:
Western blot - TLK2 antibody (ab74335)
All lanes : Anti-TLK2 antibody (ab74335) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 88 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Immunoprecipitation - TLK2 antibody (ab74335)
Detection of TLK2 by Western Blot of Immunprecipitate.
ab74335 at 1µg/ml staining TLK2 in 293 whole cell lysate immunoprecipitated using ab74335 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-TLK2 antibody (ab74335)
ab74335 has not yet been referenced specifically in any publications.
Publishing research using ab74335? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"