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Anti-TLR2 antibody [TL2.1] (ab9100)

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Overview

Product name

Anti-TLR2 antibody [TL2.1]
See all TLR2 products (32) ...

Description

Mouse monoclonal [TL2.1] to TLR2

Tested applications

ICC/IF, Flow Cyt, IHC-Fr, IP, Neutralising, WB, IHC-P, ICC, Functional Studies, IHC-Pmore details

Cross reactivity

Reacts with

Dog, Human, Cynomolgus Monkey, Rhesus monkey, Marmoset (common)

Immunogen

Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1% BSA, PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

0.2 µm filtered

Primary antibody notes

Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

Clonality

Monoclonal

Clone number

TL2.1

Isotype

IgG2a

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Transmembrane
Microbiology >> Protein >> Human Protein >> Defensin
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.

  • Immunocytochemistry - TLR2 antibody [TL2.1] (ab9100)Immunocytochemistry - TLR2 antibody [TL2.1] (ab9100) image (enlarge)

  • Flow Cytometry - TLR2 antibody [TL2.1] (ab9100)Flow Cytometry - TLR2 antibody [TL2.1] (ab9100) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

Tissue specificity

Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

Sequence similarities

Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain.

Post-translational
modifications

Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

Cellular localization

Membrane.

Target information above from: UniProt accessionO60603 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD282 antibody
  • CD282 antigen antibody
  • TIL 4 antibody
  • TIL4 antibody
  • TLR 2 antibody
  • TLR2 antibody
  • TLR2_HUMAN antibody
  • Toll like receptor 2 antibody
  • Toll like receptor 2 precursor antibody
  • Toll-like receptor 2 antibody
  • Toll/interleukin 1 receptor like 4 antibody
  • Toll/interleukin 1 receptor like protein 4 antibody
  • Toll/interleukin receptor like protein 4 antibody
  • Toll/interleukin-1 receptor-like protein 4 antibody
see all

Database links

Anti-TLR2 antibody [TL2.1] images:

  Immunocytochemistry - TLR2 antibody [TL2.1] (ab9100)

Immunocytochemistry - TLR2 antibody [TL2.1] (ab9100)

ab9100 staining TLR2 in human U937 cells by Immunocytochemistry. Cells were fixed with paraformaldehyde and blocking with 2% BSA was done for 30 minutes at 270C. Samples were incubated with primary antibody (1/50) for 1 hour at 27°C. An Alexa Fluor®568-conjugated goat polyclonal to mouse IgG (H&L) was used at dilution at 1/50 as secondary antibody.

This image is a courtesy of Mahesh Shivananjappa

See Abreview

  Flow Cytometry - TLR2 antibody [TL2.1] (ab9100)

Flow Cytometry - TLR2 antibody [TL2.1] (ab9100)

TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells

References for Anti-TLR2 antibody [TL2.1] (ab9100)

This product has been referenced in:

  • Golda A  et al. Infection with human coronavirus NL63 enhances streptococcal adherence to epithelial cells. J Gen Virol 92:1358-68 (2011).Read more (PubMed: 21325482) »
  • Alard JE  et al. TLR2 Is One of the Endothelial Receptors for {beta}2-Glycoprotein I. J Immunol : (2010). WB, ICC/IF; Human.Read more (PubMed: 20601596) »

See all 11 publications for this product

Publishing research using ab9100? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"