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Product name
Anti-TLR2 antibody
See all TLR2 products (32) ...
Description
Rabbit polyclonal to TLR2
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (Human) Blocking peptide available as ab398 69 - TLR2 peptide
Positive control
Human spleen cells and A20 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Transmembrane
Microbiology >> Protein >> Human Protein >> Defensin
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Tissue specificity
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Post-translational
modifications
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Cellular localization
Membrane.
Target information above from: UniProt accessionO60603
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD282 antibody
- CD282 antigen antibody
- TIL 4 antibody
see all
Database links
- Entrez Gene: 7097 Human
- Entrez Gene: 24088 Mouse
- Omim: 603028 Human
- SwissProt: O60603 Human
- SwissProt: Q9QUN7 Mouse
- Unigene: 519033 Human
- Unigene: 87596 Mouse
Anti-TLR2 antibody images:
Western blot - Anti-TLR2 antibody (ab24192)
All lanes : Anti-TLR2 antibody (ab24192) at 1 µg/ml
Lane 1 : A20 lysate with blocking peptide
Lane 2 : A20 lysate
Predicted band size : 90 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TLR2 antibody (ab24192)
ab24192 staining TLR2 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a citrate (pH 6.0) or EDTA (pH 9.0) buffer. Samples were incubated with primary antibody (1/400 in antibody diluent + background reducers) for 20 minutes at 25°C. An undiluted HRP-conjugated Goat polymer was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (ab24192)
ab24192 staining TLR2 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat meditated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked using 4% BSA for 30 minutes at 25°C and then incubated with ab24192 at 2µg/ml for 14 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at 10µg/ml.The enzyme for detection was alkaline phosphatase and additonal counterstaining was performed with Hematoxylin.
Image courtesy of an anonymous Abreview.
References for Anti-TLR2 antibody (ab24192)
This product has been referenced in:
- Yang B et al. Sh3pxd2b mice are a model for craniofacial dysmorphology and otitis media. PLoS One 6:e22622 (2011). IHC-P; Mouse.Read more (PubMed: 21818352) »
- Davidson LB et al. Mycobacterium abscessus glycopeptidolipid prevents respiratory epithelial TLR2 signaling as measured by HßD2 gene expression and IL-8 release. PLoS One 6:e29148 (2011). WB.Read more (PubMed: 22216191) »
See all 2 publications for this product
Publishing research using ab24192? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"