Anti-TLR3 antibody [TLR3.7] (ab12085)
Key features and details
- Mouse monoclonal [TLR3.7] to TLR3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-TLR3 antibody [TLR3.7]
See all TLR3 primary antibodies -
Description
Mouse monoclonal [TLR3.7] to TLR3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra)more details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (DDDDK-tag) corresponding to Human TLR3. Human DDDDK-tagged TLR3 stably expressed by Ba/F3 cells.
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Positive control
- Placenta and pancreas. Monocyte-derived immature dendritic cells (see paper by Matsumoto et al,2003). Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells Flow Cyt (Intra):THP-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
TLR3.7 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10.
See paper by Matsumoto et al, 2003.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody |
Notes |
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Flow Cyt (Intra)
1/10. See paper by Matsumoto et al, 2003.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
Involvement in disease
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
Domain
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
Post-translational
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
Cellular localization
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7098 Human
- Omim: 603029 Human
- SwissProt: O15455 Human
- Unigene: 657724 Human
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Alternative names
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab12085 has been referenced in 12 publications.
- Bhagat S et al. Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling. Blood Cells Mol Dis 84:102459 (2020). PubMed: 32559654
- Ishida Y et al. Candida albicans ß-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway. Infect Immun 86:N/A (2018). PubMed: 29311246
- Hwang SH et al. Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor. PLoS One 10:e0138041 (2015). PubMed: 26376485
- Yuan MM et al. TLR3 expression correlates with apoptosis, proliferation and angiogenesis in hepatocellular carcinoma and predicts prognosis. BMC Cancer 15:245 (2015). IHC . PubMed: 25884709
- Biswas I et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). PubMed: 26350442