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Product name
Anti-TLR4/MD2 Complex antibody [MTS510]
See all TLR4/MD2 Complex products (5) ...
Description
Rat monoclonal [MTS510] to TLR4/MD2 Complex
Specificity
The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2.
Tested applications
Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Toll-like receptor 4 (TLR4)/MD-2 complex of Mouse origin
Positive control
Thioglycolate-induced mouse peritoneal exudate cells (PECs)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, 150mM Sodium chloride, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
MTS510
Isotype
IgG2a
Light chain type
kappa
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Immunology >> Adaptive Immunity >> Regulatory T Cells
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab95562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Target
Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
Domain
The TIR domain mediates interaction with NOX4.
Post-translational
modifications
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
Cellular localization
Membrane.
Target information above from: UniProt accessionO00206
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Database links
- Entrez Gene: 21898 Mouse
- SwissProt: Q9QUK6 Mouse
- Unigene: 38049 Mouse
References for Anti-TLR4/MD2 Complex antibody [MTS510] (ab95562)
This product has been referenced in:
- Chabot S et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). IHC-Fr.Read more (PubMed: 16547265) »
- Mortaz E et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006).Read more (PubMed: 16413386) »
See all 4 publications for this product
Publishing research using ab95562? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"