Anti-TLR8 antibody [44C143] (ab85859)
Key features and details
- Mouse monoclonal [44C143] to TLR8
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-TLR8 antibody [44C143]
See all TLR8 primary antibodies -
Description
Mouse monoclonal [44C143] to TLR8 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TLR8 aa 750-850 conjugated to keyhole limpet haemocyanin.
Database link: Q9NR97 -
Positive control
- IHC-P: Human spleen tissue. Flow Cyt (Intra): Ramos cells. WB: 293 transfected with human TLR8.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.005% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
44C143 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 120 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Flow Cyt (Intra) |
Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 120 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Flow Cyt (Intra)
Use 0.5µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 23 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51311 Human
- Omim: 300366 Human
- SwissProt: Q9NR97 Human
- Unigene: 660543 Human
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Alternative names
- CD 288 antibody
- CD288 antibody
- CD288 antigen antibody
see all
Images
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ab85859 at 5 µg/ml staining TLR8 in formalin fixed and paraffin embedded human spleen tissue
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ab85859 at 0.5µg in 1x106 Ramos cells Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents TLR8 antibody.
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Anti-TLR8 antibody [44C143] (ab85859) at 2 µg/ml + 293 cell lysate transfected with Human TLR8
Predicted band size: 120 kDa
Observed band size: 120 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab85859 has been referenced in 3 publications.
- Ahmad R et al. Increased adipose tissue expression of TLR8 in obese individuals with or without type-2 diabetes: significance in metabolic inflammation. J Inflamm (Lond) 13:38 (2016). PubMed: 27980459
- Li Z et al. Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies. J Clin Invest 125:1081-97 (2015). PubMed: 25642772
- Luangsay S et al. Expression and functionality of Toll- and RIG-like receptors in HepaRG cells. J Hepatol 63:1077-85 (2015). PubMed: 26144659