Recombinant Anti-TLR9 antibody [EPR14964-2] (ab187148)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14964-2] to TLR9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TLR9 antibody [EPR14964-2]
See all TLR9 primary antibodies -
Description
Rabbit monoclonal [EPR14964-2] to TLR9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi, Raji and Ramos cell lysates. IHC-P: Human tonsil and breast carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14964-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 54106 Human
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- Unigene: 87968 Human
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Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Images
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All lanes : Anti-TLR9 antibody [EPR14964-2] (ab187148) at 1/1000 dilution
Lane 1 : Wild-type Raji cell lysate
Lane 2 : TLR9 knockout Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TLR9 antibody [EPR14964-2] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab187148 was shown to bind specifically to TLR9. A band was observed at 140 kDa in wild-type Raji cell lysates with no signal observed at this size in TLR9 knockout cell line ab280879 (knockout cell lysate ab282939). To generate this image, wild-type and TLR9 knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue sections labelling TLR9 with ab187148 at 1/1000 dilution. The section was incubated with ab187148 for 30 mins at room temperature. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on some immune stroma cells in human breast carcinoma tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-TLR9 antibody [EPR14964-2] (ab187148) at 1/5000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDaBlocking and dilution buffer: 5% NFDM/TBST
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue sections labelling TLR9 with ab187148 at 1/1000 dilution. The section was incubated with ab187148 for 30 mins at room temperature. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on some immune cells in human tonsil tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab187148 has not yet been referenced specifically in any publications.