You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-TLR9 antibody [5G5] (FITC) (ab58864)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-TLR9 antibody [5G5] (FITC)
See all TLR9 products (15) ...

Description

Mouse monoclonal [5G5] to TLR9 (FITC)

Conjugation

FITC

Tested applications

IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Dog, Human

Immunogen

Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc

Positive control

RAW macrophages stimulated with IFN-gamma

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

0.2 µm filtered

Clonality

Monoclonal

Clone number

5G5

Isotype

IgG2a

  • Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864)Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-P

     IHC-P: 1/50

    IHC-P: 1/50

  • ShowHide

    WB

     WB: 1/50Predicted molecu...Read more →

    WB: 1/50Predicted molecular weight: 115 kDa.

  • IHC-Fr

     IHC-Fr: 1/50

    IHC-Fr: 1/50

  • 1 Image

    Flow Cyt

     Flow Cyt: 1/50

    Flow Cyt: 1/50

  • ICC/IF

     ICC/IF: 1/50

    ICC/IF: 1/50

Target

Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Tissue specificity

Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

Sequence similarities

Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.

Cellular localization

Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.

Target information above from: UniProt accessionQ9NR96 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 289 antibody
  • CD289 antibody
  • TLR 9 antibody
  • TLR9 antibody
  • TLR9_HUMAN antibody
  • Toll like receptor 9 antibody
  • Toll like receptor 9 isoform A precursor antibody
  • Toll like receptor 9 isoform B antibody
  • Toll-like receptor 9 antibody
see all

Anti-TLR9 antibody [5G5] (FITC) images:

  Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864)

Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864)

THP1 cells were incubated with an IgG2a isotype control (A) or α-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.

References for Anti-TLR9 antibody [5G5] (FITC) (ab58864)

ab58864 has not yet been referenced specifically in any publications.

Publishing research using ab58864? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"