![Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864) image](#TLR9-Primary-antibodies-ab58864-2.jpg)
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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-TLR9 antibody [5G5] (FITC)
See all TLR9 products (15) ...
Description
Mouse monoclonal [5G5] to TLR9 (FITC)
Conjugation
FITC
Tested applications
IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Immunogen
Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
Positive control
RAW macrophages stimulated with IFN-gamma
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
0.2 µm filtered
Clonality
Monoclonal
Clone number
5G5
Isotype
IgG2a
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864)
![Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864) image](/ps/datasheet/images/58/ab58864/TLR9-Primary-antibodies-ab58864-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab58864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/50IHC-P: 1/50
WB: 1/50Predicted molecular weight: 115 kDa.
IHC-Fr
IHC-Fr: 1/50IHC-Fr: 1/50
Flow Cyt: 1/50
ICC/IF
ICC/IF: 1/50ICC/IF: 1/50
Target
Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
Target information above from: UniProt accessionQ9NR96
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Database links
- Entrez Gene: 403502 Dog
- Entrez Gene: 54106 Human
- Entrez Gene: 81897 Mouse
- Omim: 605474 Human
- SwissProt: Q5I2M8 Dog
- SwissProt: Q865B9 Dog
- SwissProt: Q9NR96 Human
- SwissProt: Q9EQU3 Mouse
see all
Anti-TLR9 antibody [5G5] (FITC) images:
Flow Cytometry - TLR9 antibody [5G5] (FITC) (ab58864)
THP1 cells were incubated with an IgG2a isotype control (A) or α-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.
References for Anti-TLR9 antibody [5G5] (FITC) (ab58864)
ab58864 has not yet been referenced specifically in any publications.
Publishing research using ab58864? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"