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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-TLR9 antibody [5G5]
See all TLR9 products (15) ...
Description
Mouse monoclonal [5G5] to TLR9
Specificity
This antibody reacts very well in mouse, but more weakly in human. There are references available which suggest the antibody works well in human, however, customer feedback suggests results in human can be inconsistant.
Tested applications
IHC-P, IHC-P, Flow Cyt, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Does not react with
Human
Positive control
RAW macrophages
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Provided as a 0.2µm filtered antibody solution
Clonality
Monoclonal
Clone number
5G5
Isotype
IgG2a
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Flow Cyt
WB
IHC-Fr
Application notes
Flow Cyt: 1/50.
IHC-Fr: 1/50.Ab16892 stains RAW macrophages and TLR9 transfected HEK293 cells.
IHC-P: Use at an assay dependent dilution.
WB: 1/50. Predicted molecular weight: 116 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
Target information above from: UniProt accessionQ9NR96
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Database links
- Entrez Gene: 81897 Mouse
- SwissProt: Q9EQU3 Mouse
- Unigene: 44889 Mouse
References for Anti-TLR9 antibody [5G5] (ab16892)
This product has been referenced in:
- Machida Het al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant : (2010).Read more (PubMed: 20181802) »
- Ilversaro et al Toll like receptor 9 agonists stimulate prostate cancer invasion in vitro The Prostate 67:774-781 (2007).Read more (PubMed: ) »
See all 2 publications for this product
Publishing research using ab16892? Please let us know so that we can cite the reference in this datasheet
![TLR9 antibody [5G5] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (16892)](/ps/datasheet/images/16/ab16892/TLR9-Primary-antibodies-ab16892-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"