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Anti-TLR9 antibody [5G5] (ab16892)

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Overview

Product name

Anti-TLR9 antibody [5G5]
See all TLR9 products (15) ...

Description

Mouse monoclonal [5G5] to TLR9

Specificity

This antibody reacts very well in mouse, but more weakly in human. There are references available which suggest the antibody works well in human, however, customer feedback suggests results in human can be inconsistant.

Tested applications

IHC-P, IHC-P, Flow Cyt, WB, IHC-Frmore details

Cross reactivity

Reacts with

Mouse

Does not react with

Human

Positive control

RAW macrophages

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Provided as a 0.2µm filtered antibody solution

Clonality

Monoclonal

Clone number

5G5

Isotype

IgG2a

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: 1/50.
IHC-Fr: 1/50.Ab16892 stains RAW macrophages and TLR9 transfected HEK293 cells.
IHC-P: Use at an assay dependent dilution.
WB: 1/50. Predicted molecular weight: 116 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Tissue specificity

Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

Sequence similarities

Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.

Cellular localization

Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.

Target information above from: UniProt accessionQ9NR96 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 289 antibody
  • CD289 antibody
  • TLR 9 antibody
  • TLR9 antibody
  • TLR9_HUMAN antibody
  • Toll like receptor 9 antibody
  • Toll like receptor 9 isoform A precursor antibody
  • Toll like receptor 9 isoform B antibody
  • Toll-like receptor 9 antibody
see all

References for Anti-TLR9 antibody [5G5] (ab16892)

This product has been referenced in:

  • Machida Het al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant : (2010).Read more (PubMed: 20181802) »
  • Ilversaro et al Toll like receptor 9 agonists stimulate prostate cancer invasion in vitro The Prostate 67:774-781 (2007).Read more (PubMed: ) »

See all 2 publications for this product

Publishing research using ab16892? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"