|
|
|
|
Western blot - TLR9 antibody (ab13928)
(enlarge)
|
|
Flow Cytometry - TLR9 antibody (ab13928)
(enlarge)
|
|
|
|
|
Product Name
|
TLR9 antibody
|
| |
See all TLR9 antibodies (15)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to TLR9
|
|
Immunogen
|
Synthetic peptide: TPGALLGLSN LTHLSLKYNN LTKVPRQLPP SLEYLLVSYNL IVKLGPEDLA NLTSLRVLDVG GNCRRCDHAPN PCIECGQKSLH LHPETFHHLSH LEGLVLKDSSL HTLN conjugated to KLH, corresponding to amino acids 200-300 of Mouse TLR9.
BLAST 'TPGALLGLSN LTHLSLKYN N LTKVPRQL PP SLEYLLV SYNL IVKLG PEDLA NLTS LRVLDVG GN CRRCDHAPN PCIECGQKSL H LHPETFHH LSH LEGLVL KDSSL HTLN ' with  or 
|
|
Reacts with
|
Ms
|
|
Does not react with
(species key)
|
Hu, Rat
|
|
Tested applications
(see key)
|
Flow Cyt, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Flow Cyt: Use 2µg for 106 cells. Raw cells or thioglycolate-elicited mouse macrophage cells.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 113 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Whole cell lysate from mouse intestinal tissue lysate.
|
|
Cellular localization
|
Cell Membrane, Type I membrane protein.
|
|
Research areas
|
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
|
|
Relevance
|
TLR9 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The gene encoding this protein is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 81897 (Mouse)
|
| |
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TLR9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for TLR9 antibody images, references, products related to ab13928 and other tools.
|
| |
TLR9 antibody images:
|
 Western blot - TLR9 antibody (ab13928)
ab13928, at a 0.5 ug/ml concentration, staining approximately 113 kDa mouse TLR9 in whole cell lysate by Western blot.
|
| |
Flow Cytometry - TLR9 antibody (ab13928)
flow cytometric analysis of ab13928 TLR9 in Raw cells at 2ug for 10^6 cells.
|
| |
References for TLR9 antibody (ab13928)
|
|
This product has been used in: (two most recent references)
Nakahira K et al. Carbon monoxide differentially inhibits TLR signaling pathways by regulating ROS-induced trafficking of TLRs to lipid rafts. J Exp Med 203:2377-89 (2006). PubMed: 17000866
Uchijima M et al. IFN-gamma overcomes low responsiveness of myeloid dendritic cells to CpG DNA. Immunol Cell Biol 83:92-5 (2005). PubMed: 15661046
See all 3 publication references for this product.
If you publish research using ab13928 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to TLR9
|
| |
TLR9 antibody - more information
|
Customer reviews (feedback) regarding TLR9 antibody |
Customer FAQs regarding TLR9 antibody |
Protocols for TLR9 antibody |
Price and availability of products related to TLR9 antibody |
| |
TLR9 antibody - related products:
|
|
|
| |
TLR9 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about TLR9 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
