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Western blot - TLR9 antibody (ab52967)
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Immunocytochemistry/ Immunofluorescence - TLR9 antibody (ab52967)
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Product Name
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TLR9 antibody
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See all TLR9 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TLR9
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Immunogen
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Synthetic peptide corresponding to 15 amino acids from an internal sequence of human TLR9.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cells, Jurkat cell lysate.
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Cellular localization
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Cell Membrane, Type I membrane protein.
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Immunoglobulins >> Receptors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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TLR9 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The gene encoding this protein is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TLR9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TLR9 antibody images, references, products related to ab52967 and other tools.
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TLR9 antibody images:
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 Western blot - TLR9 antibody (ab52967)
Lane 1 : TLR9 antibody (ab52967) at 0.5 µg/ml
Lane 2 : TLR9 antibody (ab52967) at 1 µg/ml
Lane 3 : TLR9 antibody (ab52967) at 2 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 115 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - TLR9 antibody (ab52967)
Fixed with 4%-PFA.
Immunocytochemical analysis of Jurkat cells using ab52967 at 2 µg/ml.
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Search PubMed (MEDLINE) for references to TLR9
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TLR9 antibody - more information
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Customer reviews (feedback) regarding TLR9 antibody |
Customer FAQs regarding TLR9 antibody |
Protocols for TLR9 antibody |
Price and availability of products related to TLR9 antibody |
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TLR9 antibody - related products:
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TLR9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TLR9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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