TMRE—Mitochondrial Membrane Potential Assay Kit (ab113852)
- Product nameTMRE—Mitochondrial Membrane Potential Assay KitSee all Mitochondrial Transmembrane Potential kits ...
- Product overview
ab113852 is suitable for interrogating mitochondrial membrane potential in live cells for analysis by flow cytometry, microplate spectrophotometry and fluorescent microscopy. Each assay kit contains sufficient materials for at least 200 measurements.
ab113852 uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. TMRE is a cell permeant, positively-charged, red-orange dye that readily accumulates in active mitochondria due to their relative negative charge. Depolarized or inactive mitochondria have decreased membrane potential and fail to sequester TMRE.
FCCP (carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a ionophore uncoupler of oxidative phosphorylation. Treating cells with FCCP eliminates mitochondrial membrane potential and TMRE staining. TMRE is suitable for the labeling of mitochondria in live cells and is not compatible with fixation.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 tests 1mM TMRE (in DMSO) 1 x 40µl 50mM FCCP (in DMSO) 1 x 10µl
- mitochondrial membrane potentialMS960
Our Abpromise guarantee covers the use of ab113852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
TMRE—Mitochondrial Membrane Potential Assay Kit images
Flow cytometry histogram of Jurkat cells stained with ab113852 (100nM TMRE) with (blue) or without (red) treatment with 100µM FCCP.
A: HeLa cells (adherent) were cultured on coverslips and stained with ab113852 (200nM TMRE) for 20 minutes in media, washed briefly with PBS and immediately imaged. B: Jurkat cells (suspension) were stained and washed as above and then transferred to a slide and immobilized under a coverslip for imaging.
Microplate analysis of cells showing mean fluorescent intensity +/- standard deviation from quadruplicate measurements of 400nM ab113852 (TMRE) stained Jurkat cells in a 96-well microplate +/- treatment with FCCP.
References for TMRE—Mitochondrial Membrane Potential Assay Kit (ab113852)
This product has been referenced in:
- Jandova J et al. Cyclophilin 40 alters UVA-induced apoptosis and mitochondrial ROS generation in keratinocytes. Exp Cell Res 319:750-60 (2013). Read more (PubMed: 23220213) »