Overview
- Product nameAnti-TNF Receptor I antibody [H398]See all TNF Receptor I primary antibodies ...
- DescriptionMouse monoclonal [H398] to TNF Receptor I
- Specificityab2257 reacts with the extra-cellular part of the TNF-RI. It also reacts with the soluble receptor. Be aware that the antibody competes with TNF-alpha.
- Tested applicationsIP, IHC-Fr, Flow Cyt more details
- Species reactivityReacts with: Human
- Positive controlFrozen sections: Human lymphnodes. Flow cytometry: HeLa cells. Please note: HeLa cells can differ strongly in TNF-R numbers with some populations having very low numbers.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS with 0.02% sodium azide
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Concentration information loading... - PurityIgG fraction
- Primary antibody notes The reactivity of ab2257 with cellbound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.
- Clonality Monoclonal
- Clone numberH398
- Myelomaunknown
- IsotypeIgG2a
- Light chain typeunknown
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab2257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | |
| IHC-Fr | |
| Flow Cyt |
IHC-Fr: 1/10.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
- Involvement in diseaseFamilial hibernian fever (FHF) [MIM:142680]: A hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia. Reactive amyloidosis is the main complication and occurs in 25% of cases. Note=The disease is caused by mutations affecting the gene represented in this entry.
Multiple sclerosis 5 (MS5) [MIM:614810]: A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. An intronic mutation affecting alternative splicing and skipping of exon 6 directs increased expression of isoform 4 a transcript encoding a C-terminally truncated protein which is secreted and may function as a TNF antagonist. - Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats. - DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. - Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. - Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
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Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
Target information above from: UniProt accession
P19438
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD120a antibodyCD120a antigen antibodyFPF antibody
- MGC19588 antibodyp55 antibodyp55 R antibodyp55-R antibodyp55R antibodyp60 antibodyTBP1 antibodyTBPI antibodyTNF R antibodyTNF R I antibodyTNF R1 antibodyTNF R55 antibodyTNF-R1 antibodyTNF-RI antibodyTNFAR antibodyTNFR 1 antibodyTNFR I antibodyTNFR-I antibodyTNFR1 antibodyTNFR55 antibodyTNFR60 antibodyTNFRI antibodyTNFRSF1a antibodyTNFRSF1a antibodyTNR1A_HUMAN antibodyTumor necrosis factor alpha receptor antibodyTumor necrosis factor binding protein 1 antibodyTumor necrosis factor receptor 1 antibodyTumor necrosis factor receptor superfamily, member 1A antibodyTumor necrosis factor receptor type 1 antibodyTumor necrosis factor receptor type I antibodyTumor necrosis factor-binding protein 1 antibody
see all
References for Anti-TNF Receptor I antibody [H398] (ab2257)
This product has been referenced in:
- Greco SJ & Rameshwar P Enhancing effect of IL-1alpha on neurogenesis from adult human mesenchymal stem cells: implication for inflammatory mediators in regenerative medicine. J Immunol 179:3342-50 (2007). WB ; Human . Read more (PubMed: 17709551) »
- Grell M et al. TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 12:143-8 (1993). Read more (PubMed: 8394147) »
