Anti-TNF Receptor I antibody [H398] (ab2257)


  • Product nameAnti-TNF Receptor I antibody [H398]
    See all TNF Receptor I primary antibodies
  • Description
    Mouse monoclonal [H398] to TNF Receptor I
  • Specificityab2257 reacts with the extra-cellular part of the TNF-RI. It also reacts with the soluble receptor. Be aware that the antibody competes with TNF-alpha.
  • Tested applicationsIP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Frozen sections: Human lymphnodes. Flow cytometry: HeLa cells. Please note: HeLa cells can differ strongly in TNF-R numbers with some populations having very low numbers.
  • General notes

    The reactivity of ab2257 with cellbound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.



Our Abpromise guarantee covers the use of ab2257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr 1/10.
Flow Cyt 1/10.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in diseaseFamilial hibernian fever
      Multiple sclerosis 5
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120a antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55-R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    References for Anti-TNF Receptor I antibody [H398] (ab2257)

    This product has been referenced in:
    • Greco SJ & Rameshwar P Enhancing effect of IL-1alpha on neurogenesis from adult human mesenchymal stem cells: implication for inflammatory mediators in regenerative medicine. J Immunol 179:3342-50 (2007). WB ; Human . Read more (PubMed: 17709551) »
    • Grell M  et al. TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 12:143-8 (1993). Read more (PubMed: 8394147) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (brain endothelial cells)
    Loading amount 10 µg
    Specification brain endothelial cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15%

    Abcam user community

    Verified customer

    Submitted May 30 2007

    Thank you for your enquiry. I am contacting the person who made this data sheet to first confirm that the dilution is 1/10 because that seems rather concentrated to me. I will get back to you as soon as I hear back. Thank you for your patience. I h...

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    Thank you for your question concerning the ab2257. Concerning the source of immunizing protein used to generate this monoclonal we have received the following information: About 220µg affinity-purified receptor material from HL-60 cells containing ...

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    Thank you for your enquiry. Unfortunately this information is not available. If you have any additional questions, please contact us again.

    In principle it could be used for the precipitation of soluble TNF Receptor 1 in serum, however this has not been specifically tested to our knowledge.

    Thanks for your email. Ab2257 is not avaialble conjugated and we are unable to do custom conjugation. If you have any further questions, please contact us again.